Elispot assay kit

Mabtech's platform of high quality monoclonal antibodies enables research on your own terms. The limit of detection typically achieved can be 1 incells. The detection antibody is either biotinylated and followed by a streptavidin-enzyme conjugate or the antibody is directly conjugated to an enzyme.

The last step in the assay is the addition of a substrate yielding a colored zone 'spot'which reveals the site of cytokine secretion. By using a substrate with a precipitating rather than a soluble product, the end result is visible spots on the surface.

After an appropriate incubation time, cells are removed and the secreted molecule is detected using a detection antibody in a similar procedure to that employed by the ELISA.

Elispot assay kit

The ELISPOT assay is currently being used increasingly for the quantitative assessment of peptide reactive T lymphocytes from PBMC in infectious diseases 8 , 9 , in the course of vaccination trials aimed at the induction of tumor-specific T cells 10 , 11 , or in the assessment of immune-mediated pathogenesis of autoimmune diseases 12 , The assay is therefore easy to perform and allows rapid analysis of a large number of samples. The ELISpot technique is not limited to measurement of cytokines; it is also suitable for almost any secreted protein where single-cell analysis is of interest. The detection antibody is either biotinylated and followed by a streptavidin-enzyme conjugate or the antibody is directly conjugated to an enzyme. Sensitive The ELISpot assay captures the presence of cytokines immediately after secretion, in contrast to measurements that are skewed by receptor binding or protease degradation. The assay is considered as one of the most sensitive cellular assays available. The method was originally developed to quantify immunoglobulin-secreting cells. Kit contents:. The assay is considered as one of the most sensitive cellular assays available. Mabtech's platform of high quality monoclonal antibodies enables research on your own terms. Each spot corresponds to an individual cytokine-secreting cell. The ELISPOT assay is able to monitor the stimulatory effects of candidate adjuvants on cellular immune responses to co-administered vaccine proteins 14 , By using a substrate with a precipitating rather than a soluble product, the end result is visible spots on the surface. Mabtech has worked extensively for more than 20 to 30 years to optimize the ELISpot protocol.

Mabtech has worked extensively for more than 20 to 30 years to optimize the ELISpot protocol. Proteins, such as cytokines, that are secreted by the cells will be captured by the specific antibodies on the surface. The ELISPOT assay can be used to determine effects of drugs, chemicals or other compounds on cytokine secretion in vitro, thereby providing data on their putative modulatory effects on immune function in vivo 45.

The assay is considered as one of the most sensitive cellular assays available.

dual color elispot
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ELISpot Assay Principle: R&D Systems